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验证数据展示

  • WB analysis using 31717-1-AP

    WB analysis using 31717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31717-1-AP (SLAMF3/CD229 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

CD229/SLAMF3 Polyclonal antibody

CD229/SLAMF3 Polyclonal Antibody for WB, ELISA
Cat No. 31717-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

SLAMF3, hly9, Cell surface molecule Ly-9, Cell surface molecule Ly 9, CDABP0070

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU266 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31717-1-AP targets CD229/SLAMF3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CD229/SLAMF3 fusion protein Ag34617 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 9
别名 SLAMF3, hly9, Cell surface molecule Ly-9, Cell surface molecule Ly 9, CDABP0070
观测分子量90 kDa, 110 kDa
GenBank蛋白编号BC121049
基因名称 LY9
Gene ID (NCBI) 4063
RRIDAB_3670094
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBG7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).

实验方案

Product Specific Protocols
WB protocol for CD229/SLAMF3 antibody 31717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols