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SKAP55 Polyclonal antibody

SKAP55 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14724-1-AP

Print datasheet

Synonyms

pp55, SCAP1, SKAP 55, SKAP1, SKAP55

验证数据展示

  • WB analysis of mouse thymus using 14724-1-AP

    WB analysis of mouse thymus using 14724-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14724-1-AP

    IHC staining of human spleen using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14724-1-AP

    IHC staining of human spleen using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14724-1-AP targets SKAP55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SKAP55 fusion protein Ag6467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name src kinase associated phosphoprotein 1
Synonyms pp55, SCAP1, SKAP 55, SKAP1, SKAP55
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC047870
Gene Symbol SKAP1
Gene ID (NCBI) 8631
RRIDAB_10858320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKAP55 antibody 14724-1-APDownload protocol
IHC protocol for SKAP55 antibody 14724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols