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验证数据展示

  • WB analysis using 12496-1-AP (same clone as 12496-1-PBS)

    WB analysis using 12496-1-AP (same clone as 12496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 12496-1-AP (same clone as 12496-1-PBS)

    IHC staining of human stomach using 12496-1-AP (same clone as 12496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    IF Staining of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12496-1-AP (same clone as 12496-1-PBS)

    IF Staining of HepG2 using 12496-1-AP (same clone as 12496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SHC1 Polyclonal antibody, PBS Only

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12496-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12496-1-PBS targets SHC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3165

Product name: Recombinant human SHC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 175-474 aa of BC014158

Sequence: RACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SHC (Src homology 2 domain containing) transforming protein 1
别名 SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1
计算分子量 66 kDa
观测分子量 66 kDa, 52 kDa, 46 kDa
GenBank蛋白编号BC014158
基因名称 SHC1
Gene ID (NCBI) 6464
RRIDAB_2187889
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29353
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

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