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验证数据展示

  • WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

    IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

    IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

SGLT1 Polyclonal antibody, PBS Only

SGLT1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 30861-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

SLC5A1, High affinity sodium-glucose cotransporter

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30861-1-PBS targets SGLT1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29338

Product name: Recombinant human SLC5A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 564-643 aa of NM_000343

Sequence: RNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 5 (sodium/glucose cotransporter), member 1
别名 SLC5A1, High affinity sodium-glucose cotransporter
计算分子量 73 kDa
观测分子量 73 kDa
GenBank蛋白编号NM_000343
基因名称 SGLT1
Gene ID (NCBI) 6523
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13866
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).

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