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验证数据展示

  • WB analysis using 32098-1-AP

    WB analysis using 32098-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32098-1-AP (SEC14L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 32098-1-AP

    IF Staining of HeLa using 32098-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC14L3 antibody (32098-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SEC14L3 Polyclonal antibody

SEC14L3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32098-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Tocopherol-associated protein 2, TAP2, SEC14-like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue, rat lung tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32098-1-AP targets SEC14L3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEC14L3 fusion protein Ag36792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC14-like 3 (S. cerevisiae)
别名 Tocopherol-associated protein 2, TAP2, SEC14-like protein 3
计算分子量 46 kDa
观测分子量45 kDa
GenBank蛋白编号BC069641
基因名称 SEC14L3
Gene ID (NCBI) 266629
RRIDAB_3670189
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UDX4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC14L3 is highly similar to the protein encoded by the Saccharomyces cerevisiae SEC14 gene. The SEC14 protein is a phophatidylinositol transfer protein that is essential for biogenesis of Golgi-derived transport vesicles, and thus is required for the export of yeast secretory proteins from the Golgi complex. The specific function of this protein has not yet been determined. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

实验方案

Product Specific Protocols
WB protocol for SEC14L3 antibody 32098-1-APDownload protocol
IF protocol for SEC14L3 antibody 32098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols