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验证数据展示

  • IF Staining of human astrocytes using 15146-1-AP (same clone as 15146-1-PBS)

    IF Staining of human astrocytes using 15146-1-AP (same clone as 15146-1-PBS)

    Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

  • WB analysis using 15146-1-AP (same clone as 15146-1-PBS)

    WB analysis using 15146-1-AP (same clone as 15146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 15146-1-AP (same clone as 15146-1-PBS)

    IHC staining of human malignant melanoma using 15146-1-AP (same clone as 15146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 15146-1-AP (same clone as 15146-1-PBS)

    IHC staining of human malignant melanoma using 15146-1-AP (same clone as 15146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    IF Staining of mouse brain using 15146-1-AP (same clone as 15146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S100B antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

  • FC experiment of A375 using 15146-1-AP (same clone as 15146-1-PBS)

    FC experiment of A375 using 15146-1-AP (same clone as 15146-1-PBS)

    1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human S100 Beta (15146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 15146-1-PBS in a different storage buffer formulation.

S100B Polyclonal antibody, PBS Only

S100B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 15146-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.

产品信息

15146-1-PBS targets S100B in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7440

Product name: Recombinant human S100B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-92 aa of BC001766

Sequence: MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein B
别名 S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC001766
基因名称 S100 Beta
Gene ID (NCBI) 6285
RRIDAB_2254244
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04271
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100B belongs to the EF-hand calcium binding proteins and is found primarily in astrocytes in the central nervous system (CNS). S100B has a variety of functions, including calcium homeostasis, cell proliferation, differentiation, migration, and survival, as well as neurite outgrowth and regeneration.

 1. What is the molecular weight of S100B?

S100B protein is composed of non-covalently linked homodimers of 11 kDa size.

2. What is the subcellular localization of S100B?

S100B localizes to the nucleus and cytoplasm, associating with intracellular membranes, the centrosomes, microtubules, and type III intermediate filaments (PMID: 19110011). Additionally, it can be released from astrocytes into the extracellular space and can enter the bloodstream.

3. What is the expression pattern of S100B?

S100B is predominantly expressed in astrocytes and maturing oligodendrocytes but is also present in other cell types such as kidney epithelial cells, neural progenitor cells, pituicytes, ependymocytes, chondrocytes, adipocytes, melanocytes, Langerhans cells, dendritic cells, certain lymphocyte subpopulations, skeletal myofibers, myoblasts, and muscle satellite cells (PMID: 19110011). S100B is a commonly used marker of Schwann cells and reactive astrocytes in ICC, IHC, and WB applications.

4. What is the diagnostic use of S100B in the clinic?

S100B is naturally secreted by astrocytes into the extracellular space and low amounts of S100B can pass through the brain-blood barrier and enter the bloodstream. Elevated levels of S100B in the serum are observed in patients with traumatic head injuries, as well as in patients suffering from neurodegenerative diseases (PMID: 30144068). This increase in S100B levels is attributed to the elevated secretion of S100B protein from astrocytes as part of the physiological response to the injury, as well as to the physical damage of astrocytes and increased blood-brain barrier permeability.


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