验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14909-1-AP targets RPS26 in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, PLA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPS26 fusion protein Ag6706 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S26 |
| 别名 | Small ribosomal subunit protein eS26, ribosomal protein S26, 40S ribosomal protein S26 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 18-21 kDa |
| GenBank蛋白编号 | BC002604 |
| 基因名称 | RPS26 |
| Gene ID (NCBI) | 6231 |
| RRID | AB_2180361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62854 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IHC protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IF protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IP protocol for RPS26 antibody 14909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Nat Commun Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity. | ||
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Oncogene The ribosomal protein S26 regulates p53 activity in response to DNA damage.
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