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验证数据展示

  • WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 29803-1-AP (same clone as 29803-1-PBS)

    IF Staining of PC-3 using 29803-1-AP (same clone as 29803-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 29803-1-AP (same clone as 29803-1-PBS)

    IHC staining of rat cerebellum using 29803-1-AP (same clone as 29803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RGS12 antibody included

RGS12 Polyclonal antibody, PBS Only

RGS12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29803-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Regulator of G-protein signaling 12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RGS12 Ready-To-Use IHC Kit. RGS12 primary antibody included.

产品信息

29803-1-PBS targets RGS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30731

Product name: Recombinant human RGS12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 833-962 aa of NM_198229

Sequence: LAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of G-protein signaling 12
别名 Regulator of G-protein signaling 12
计算分子量 156 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_198229
基因名称 RGS12
Gene ID (NCBI) 6002
RRIDAB_2935482
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14924
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

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