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验证数据展示

  • IF Staining of HeLa using CL488-84892

    IF Staining of HeLa using CL488-84892

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RFC2 antibody (CL488-84892, Clone: 242503E12 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated RFC2 Recombinant antibody

RFC2 Recombinant Antibody for IF/ICC
Cat No. CL488-84892

产品说明书

CloneNo. 242503E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

242503E12, A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84892-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84892 targets RFC2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 RFC2 fusion protein Ag0663 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication factor C (activator 1) 2, 40kDa
别名 242503E12, A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2
计算分子量 39 kDa
GenBank蛋白编号BC002813
基因名称 RFC2
Gene ID (NCBI) 5982
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35250
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RFC2 antibody CL488-84892Download protocol
Standard Protocols
Click here to view our Standard Protocols