验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31209-1-AP targets RENBP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RENBP fusion protein Ag35056 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | renin binding protein |
| 别名 | AGE, EC:5.1.3.8, GlcNAc 2-epimerase, N-acetyl-D-glucosamine 2-epimerase, N-acylglucosamine 2-epimerase |
| 计算分子量 | 49 kDa |
| 观测分子量 | 29 kDa, 45-49 kDa |
| GenBank蛋白编号 | BC015558 |
| 基因名称 | RENBP |
| Gene ID (NCBI) | 5973 |
| RRID | AB_3669902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P51606 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Renin binding protein (RENBP) was originally identified as a protein in the kidney that was capable of binding renin in renal homogenates giving rise to a complex designated high molecular weight renin (PMID: 12866502). Studies have shown that human RnBP is the enzyme N-acetyl-D-glucosamine (GlcNAc) 2-epimerase (PMID: 12866502). RENBP has 3 isoforms produced by alternative splicing and alternative initiation with molecular weights of 29, 48, and 49 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RENBP antibody 31209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |