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验证数据展示

  • WB analysis using 31527-1-AP

    WB analysis using 31527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31527-1-AP (RBP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 31527-1-AP

    IHC staining of human small intestine using 31527-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 31527-1-AP (RBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 31527-1-AP

    IHC staining of human small intestine using 31527-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 31527-1-AP (RBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBP2 Polyclonal antibody

RBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31527-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CRBP2, Retinol binding protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse small intestine tissue, rat small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31527-1-AP targets RBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35801

Product name: Recombinant human RBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-134 aa of NM_004164.2

Sequence: RKIAVRLTQTKVIDQDGDNFKTKTTSTFRNYDVDFTVGVEFDEYTKSLDNRHVKALVTWEGDVLVCVQKGEKENRGWKQWIEGDKLYLELTCGDQVCRQVFKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol binding protein 2, cellular
别名 CRBP2, Retinol binding protein 2
计算分子量16 kDa
观测分子量 16 kDa
GenBank蛋白编号NM_004164.2
基因名称 RBP2
Gene ID (NCBI) 5948
RRIDAB_3670022
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP50120
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinol-binding protein 2 (RBP2) also known as CRBP2, CRBP-II, is an abundant 134-residue protein confined largely to the small intestinal enterocyte. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A(PMID: 3029082).

实验方案

Product Specific Protocols
IHC protocol for RBP2 antibody 31527-1-APDownload protocol
WB protocol for RBP2 antibody 31527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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