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验证数据展示

  • WB analysis using 26997-1-AP

    WB analysis using 26997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26997-1-AP (RASGRP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26997-1-AP

    IF Staining of HepG2 using 26997-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASGRP1 antibody (26997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RASGRP1 Polyclonal antibody

RASGRP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26997-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Ras guanyl releasing protein, hRasGRP1, CalDAG-GEFII, CALDAG GEFII, CALDAG GEFI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26997-1-AP targets RASGRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25725

Product name: Recombinant human RASGRP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 678-797 aa of BC109297

Sequence: QPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVLAQMEQGDCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS guanyl releasing protein 1 (calcium and DAG-regulated)
别名 Ras guanyl releasing protein, hRasGRP1, CalDAG-GEFII, CALDAG GEFII, CALDAG GEFI
计算分子量 797 aa, 90 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC109297
基因名称 RASGRP1
Gene ID (NCBI) 10125
RRIDAB_3669579
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95267
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGRP1 (RAS guanyl-releasing protein 1) is a Ras guanine nucleotide exchange factor, and an essential regulator of lymphocyte receptor signaling (PMID: 33065764). RasGRP1 is a bifunctional regulator that promotes acute inflammation and inhibits inflammation-associated cancer (PMID: 36385095). RasGRP1, a downstream target gene of VEGF, regulates the development, homeostasis, and differentiation of T cells (PMID: 37429143).

实验方案

Product Specific Protocols
IF protocol for RASGRP1 antibody 26997-1-APDownload protocol
WB protocol for RASGRP1 antibody 26997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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