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验证数据展示

  • WB analysis using 27168-1-AP

    WB analysis using 27168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27168-1-AP (RANBP3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 27168-1-AP

    WB analysis of Jurkat using 27168-1-AP

    WB result of RANBP3 antibody (27168-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP3 transfected Jurkat cells.

  • IHC staining of mouse testis using 27168-1-AP

    IHC staining of mouse testis using 27168-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27168-1-AP (RANBP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 27168-1-AP

    IF Staining of HepG2 using 27168-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RANBP3 antibody (27168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  • KD/KO验证

RANBP3 Polyclonal antibody

RANBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27168-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Ran-binding protein 3, RAN binding protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27168-1-AP targets RANBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26000

Product name: Recombinant human RANBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-176 aa of BC004349

Sequence: SPEGGEDSDREDGNYCPPVKRERTSSLTQFPPSQSEERSSGFRLKPPTLIHGQAPSAGLPSQKPKEQQRSVLRPAVLQAPQPKALSQTVPSSGTNGVSLPADCTGAVPAASPDTAAWRSPSEAADEVCALEEKEPQKNESSNAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAN binding protein 3
别名 Ran-binding protein 3, RAN binding protein 3
计算分子量 60 kDa
观测分子量 70 kDa
GenBank蛋白编号BC004349
基因名称 RANBP3
Gene ID (NCBI) 8498
RRIDAB_3669586
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Z4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RANBP3 (Ran-binding protein 3) is also named as Rab escort protein 1 (REP-1). RanBP3 is a RanGTP‐binding protein that has been reported to enhance nuclear export of active β‐catenin, independent of its role as a CRM1‐associated nuclear export cofactorl (PMID: 9637251). RanBP3 has been reported to be a crucial downstream molecule of MAPK-ERK and PI3K-AKT signal pathways (PMID: 18331709). RanBP3 is a tumor suppressor in CRC and is associated with patient survival (PMID: 36270974). RanBP3 has been reported as a potential regulator in melanoma biology, hepatocellular carcinoma, prostate cancer, and ovarian cancer, and RanBP3 is highly expressed in CML Cells (PMID: 34504783).

实验方案

Product Specific Protocols
IF protocol for RANBP3 antibody 27168-1-APDownload protocol
IHC protocol for RANBP3 antibody 27168-1-APDownload protocol
WB protocol for RANBP3 antibody 27168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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