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验证数据展示

  • WB analysis of MCF-7 using 31303-1-AP

    WB analysis of MCF-7 using 31303-1-AP

    WB result of RAB21 antibody (31303-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB21 transfected MCF-7 cells.

  • WB analysis using 31303-1-AP

    WB analysis using 31303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31303-1-AP (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31303-1-AP

    WB analysis using 31303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31303-1-AP (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 31303-1-AP

    IF Staining of MCF-7 using 31303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RAB21 antibody (31303-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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RAB21 Polyclonal antibody

RAB21 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31303-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA0118, Ras related protein Rab 21

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31303-1-AP targets RAB21 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35291

Product name: Recombinant human RAB21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 115-225 aa of NM_014999

Sequence: KELRKMLGNEICLCIVGNKIDLEKERHVSIQEAESYAESVGAKHYHTSAKQNKGIEELFLDLCKRMIETAQVDERAKGNGSSQPGTARRGVQIIDDEPQAQTSGGGCCSSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB21, member RAS oncogene family
别名 KIAA0118, Ras related protein Rab 21
计算分子量24 kDa
观测分子量24 kDa
GenBank蛋白编号NM_014999
基因名称 RAB21
Gene ID (NCBI) 23011
RRIDAB_3669940
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UL25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPase Rab21 is a ubiquitously expressed and has recently been shown to associate with the α tails of several integrins via the shared conserved membrane proximal sequence, thus regulating cell adhesion and migration via controlling the endo/exocytic trafficking of most integrin heterodimers (PMID: 18804435). Rab21 is required for the pruning of the immature neurites during the multipolar-to-bipolar transition (PMID: 36683567). Knock down of Rab21 impairs integrin-mediated cell adhesion and motility, whereas its overexpression stimulates cell migration and cancer cell adhesion to collagen and human bone (PMID: 16754960).

实验方案

Product Specific Protocols
IF protocol for RAB21 antibody 31303-1-APDownload protocol
WB protocol for RAB21 antibody 31303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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