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验证数据展示

  • WB analysis using 13169-1-AP (same clone as 13169-1-PBS)

    WB analysis using 13169-1-AP (same clone as 13169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13169-1-AP (QKI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13169-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 13169-1-AP (same clone as 13169-1-PBS)

    IHC staining of human stomach cancer using 13169-1-AP (same clone as 13169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13169-1-AP (QKI antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13169-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 13169-1-AP (same clone as 13169-1-PBS)

    IP experiment of K-562 using 13169-1-AP (same clone as 13169-1-PBS)

    IP result of anti-QKI (IP:13169-1-AP, 4ug; Detection:13169-1-AP 1:400) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 13169-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

QKI Polyclonal antibody, PBS Only

QKI Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13169-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13169-1-PBS targets QKI in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3822

Product name: Recombinant human QKI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC019917

Sequence: MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 quaking homolog, KH domain RNA binding (mouse)
别名
计算分子量 341 aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC019917
基因名称 QKI
Gene ID (NCBI) 9444
RRIDAB_2173138
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PU8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

QKI (Quaking) is RNA-binding protein that plays a central role in myelinization (PMID: 16641098). It regulates pre-mRNA splicing, mRNA export and protein translation (PMID: 16641098). It acts upstream of or within several processes, including long-chain fatty acid biosynthetic process, positive regulation of macromolecule metabolic process and vasculogenesis. QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism. It is expressed in the frontal cortex of brain (PMID: 16342280).

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