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验证数据展示

  • WB analysis of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    WB analysis of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    Western blot analysis of anti-polyglutamine antibody (MW1) binding to huntingtin exon 1 fusion proteins with variable numbers of glutamines. MW1 bound to huntingtin exon 1 proteins with normal and expanded polyQ repeats but did not bind the TRX tag control. (Owens, Gwen E et al. J Mol Biol. 2015 Jul 31;427(15):2507-2519.) This data was developed using the same antibody clone with 65239-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    ELISA experiment of Recombinant protein using 65239-1-Ig (same clone as 65239-1-PBS)

    Indirect ELISA was carried out by coating polyglutamine containing ASCL1 fusion protein (Cat.NO. Ag26337, containing polyglutamine region) and GST fusion protein expressed from the same vector (Cat.NO. Ag25094, for control) at 70 ng/well followed by blocking and adding serial diluted Polyglutamine mouse monoclonal antibody 65239-1-Ig. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 65239-1-PBS in a different storage buffer formulation.

Polyglutamine Monoclonal antibody, PBS Only

Polyglutamine Monoclonal Antibody for WB, ELISA, Indirect ELISA
Cat No. 65239-1-PBS

产品说明书

CloneNo. MW1

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

n/a

应用

WB, ELISA, Indirect ELISA

别名

Glutamine repeats, polyQ

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

65239-1-PBS targets Polyglutamine in WB, ELISA, Indirect ELISA applications and shows reactivity with n/a samples.

经测试应用 WB, ELISA, Indirect ELISA Application Description
经测试反应性 n/a
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 Polyglutamine
别名 Glutamine repeats, polyQ
GenBank蛋白编号N/A
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Huntington's disease is a neurodegenerative disorder caused by the expansion of a polyglutamine (polyQ) repeat in the N-terminal portion of huntingtin protein to a length above 35-40 units (PMID: 26047735; 19507258). The mutational expansion of polyglutamine above a critical length causes a toxic gain of function in huntingtin and results in neuronal death. In the course of the disease, expanded huntingtin is proteolyzed, becomes abnormally folded, and accumulates in oligomers, fibrils, and microscopic inclusions (PMID: 25336039). The anti-polyglutamine (polyQ) antibody MW1 specifically binds the polyQ domain of huntingtin exon 1. On western blot, the MW1 clone strongly prefers to bind to the expanded polyQ repeat form of Htt, displaying no detectable binding to normal huntingtin (PMID: 11719267).