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Plakophilin 2 Polyclonal antibody

Plakophilin 2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 26479-1-AP

Print datasheet

Synonyms

ARVD9, PKP2, plakophilin 2

验证数据展示

  • WB analysis using 26479-1-AP

    WB analysis using 26479-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26479-1-AP

    IP experiment of HepG2 using 26479-1-AP

    IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.

经过测试的应用

Positive WB detected inCaco-2 cells, HepG2 cells, rat heart tissue
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26479-1-AP targets Plakophilin 2 in WB, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, rat
Immunogen Plakophilin 2 fusion protein Ag24155 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plakophilin 2
Synonyms ARVD9, PKP2, plakophilin 2
Calculated Molecular Weight 837 aa, 93 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC094762
Gene Symbol PKP2
Gene ID (NCBI) 5318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99959
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakophilin 2 also known as PKP2, belongs to the beta-catenin family. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10828611). Mutations in PKP2 are associated with Arrhythmogenic right ventricular dysplasia, familial, 9 (ARVD9)(PMID: 15489853).

实验方案

Product Specific Protocols
WB protocol for Plakophilin 2 antibody 26479-1-APDownload protocol
IP protocol for Plakophilin 2 antibody 26479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols