验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells |
| Positive FC (Intra) detected in | λ phosphatase treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80103-2-RR targets Phospho-GRB10 (Ser476) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | growth factor receptor-bound protein 10 |
| 别名 | GRB10 (Ser476), p-GRB10, p-GRB10 (Ser476), p-GRB10(Ser476), p-GRB10-Ser476 |
| 计算分子量 | 536aa,61 kDa; 594aa,67 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC024285 |
| 基因名称 | GRB10 |
| Gene ID (NCBI) | 2887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13322 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-GRB10 (Ser476) antibody 80103-2-RR | Download protocol |
| WB protocol for Phospho-GRB10 (Ser476) antibody 80103-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
