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PTPN18 Polyclonal antibody

PTPN18 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17551-1-AP

Print datasheet

Synonyms

BDP1, Brain derived phosphatase, PTP HSCF, PTPN18

验证数据展示

  • WB analysis of Jurkat using 17551-1-AP

    WB analysis of Jurkat using 17551-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17551-1-AP (PTPN18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17551-1-AP

    IHC staining of human brain using 17551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17551-1-AP (PTPN18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17551-1-AP

    IHC staining of human brain using 17551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17551-1-AP (PTPN18 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, human brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17551-1-AP targets PTPN18 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen PTPN18 fusion protein Ag11704 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
Synonyms BDP1, Brain derived phosphatase, PTP HSCF, PTPN18
Calculated Molecular Weight 460 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC052800
Gene Symbol PTPN18
Gene ID (NCBI) 26469
RRIDAB_2284599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN18(Tyrosine-protein phosphatase non-receptor type 18) is also named as BDP1 and belongs to the protein-tyrosine phosphatase family. The PTPN18 cDNA encodes a deduced 459-amino acid protein with no transmembrane domain and a calculated molecular weight of 50 kD. PTPN18 has a PEST motif and shares the greatest homology with members of the PTP-PEST gene family. Northern blot analysis indicated that PTPN18 is expressed not only in brain, but also in colon and several different tumor-derived cell lines.(PMID:8950995). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PTPN18 antibody 17551-1-APDownload protocol
IHC protocol for PTPN18 antibody 17551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Physiol Biochem

Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.

Authors - Guangyan Zhangyuan
View Article
humanWB

Clin Exp Pharmacol Physiol

Downregulation of PTPN18 can inhibit proliferation and metastasis and promote apoptosis of endometrial cancer.

Authors - Junhong Cai
View Article