验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17551-1-AP targets PTPN18 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | PTPN18 fusion protein Ag11704 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, non-receptor type 18 (brain-derived) |
| 别名 | BDP1, Brain derived phosphatase, PTP HSCF, PTPN18 |
| 计算分子量 | 460 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC052800 |
| 基因名称 | PTPN18 |
| Gene ID (NCBI) | 26469 |
| RRID | AB_2284599 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99952 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPN18(Tyrosine-protein phosphatase non-receptor type 18) is also named as BDP1 and belongs to the protein-tyrosine phosphatase family. The PTPN18 cDNA encodes a deduced 459-amino acid protein with no transmembrane domain and a calculated molecular weight of 50 kD. PTPN18 has a PEST motif and shares the greatest homology with members of the PTP-PEST gene family. Northern blot analysis indicated that PTPN18 is expressed not only in brain, but also in colon and several different tumor-derived cell lines.(PMID:8950995). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPN18 antibody 17551-1-AP | Download protocol |
| IHC protocol for PTPN18 antibody 17551-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma. | ||
Mol Cancer Res Hepatitis B virus X protein represses expression of tumor suppressor PTPN18 in hepatocellular carcinoma | ||
Clin Exp Pharmacol Physiol Downregulation of PTPN18 can inhibit proliferation and metastasis and promote apoptosis of endometrial cancer. |
