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PYK2 Polyclonal antibody

PYK2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10044-2-AP

Print datasheet

Synonyms

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

验证数据展示

  • WB analysis of mouse brain using 10044-2-AP

    WB analysis of mouse brain using 10044-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 10044-2-AP

    IHC staining of human medulloblastoma using 10044-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 10044-2-AP (PYK2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IHC detected inhuman medulloblastoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10044-2-AP targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen PYK2 fusion protein Ag0002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PTK2B protein tyrosine kinase 2 beta
Synonyms CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC036651
Gene Symbol PTK2B
Gene ID (NCBI) 2185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14289
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several introcellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis.

发表文章

SpeciesApplicationTitle
ratWB

Herz

Ginsenoside-Rb3 inhibits endothelial-mesenchymal transition of cardiac microvascular endothelial cells.

Authors - L Yang
View Article