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验证数据展示

  • WB analysis of mouse brain using 10044-2-AP

    WB analysis of mouse brain using 10044-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 10044-2-AP

    IHC staining of human medulloblastoma using 10044-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 10044-2-AP (PYK2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PTK2B antibody included

PYK2 Polyclonal antibody

PYK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10044-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IHC detected inhuman medulloblastoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTK2B Ready-To-Use IHC Kit. PTK2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10044-2-AP targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性rat
免疫原

CatNo: Ag0002

Product name: Recombinant human PTK2B protein

Source: e coli.-derived, Unknow

Tag: N/A

Domain: Gst-PYK1-C of BC036651

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PTK2B protein tyrosine kinase 2 beta
别名 CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK
计算分子量 120 kDa
观测分子量 116 kDa
GenBank蛋白编号BC036651
基因名称 PTK2B
Gene ID (NCBI) 2185
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14289
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several introcellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis.

发表文章

SpeciesApplicationTitle
ratWB

Herz

Ginsenoside-Rb3 inhibits endothelial-mesenchymal transition of cardiac microvascular endothelial cells.

Authors - L Yang
View Article