验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26473-1-AP targets PTCD2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag24115 Product name: Recombinant human PTCD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 153-388 aa of NM_001284403 Sequence: MKDQHLRGFFSDSTSFNILMDMLFIKGKYKSALQVLIEMKNQDVKFTKDTYVLAFAICYKLNSPESFKICTTLREEALLKGEILSRRASCFAVALALNQNEMAKAVSIFSQIMNPESIACINLNIIIHIQSNMLENLIKTLKNAAEGNLSKFVKRHVFSEEVLAKVREKVKDVPALVAKFDEIYGTLHITGQVTTDSLDAVLCHTPRDRKSHTLLLNKRMVSRRTFQPLSQSLLAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pentatricopeptide repeat domain 2 |
| 别名 | Pentatricopeptide repeat-containing protein 2, mitochondrial |
| 观测分子量 | 33 kDa, 50 kDa |
| GenBank蛋白编号 | NM_001284403 |
| 基因名称 | PTCD2 |
| Gene ID (NCBI) | 79810 |
| RRID | AB_3669538 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WV60 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTCD2 (Pentatricopeptide repeat-containing protein 2), a mysterious protein whose function was unknown despite the available data. Its mitochondrial localization and PPR family membership suggested its possible biological role in regulation of mitochondrial gene expression. (PMID: 36430722) This antibody recognizes three isomers of PTCD2, including isomer 1 (≈ 44 kDa), isomer 2 (≈ 24 kDa) and isomer 3 (≈ 31 kDa). Since the protein has two post-translational modifications, phosphorylation and ubiquitination, the actual measured molecular weight will be larger than the predicted value.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCD2 antibody 26473-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
