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验证数据展示

  • WB analysis using 68641-1-Ig

    WB analysis using 68641-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68641-1-Ig (PSMB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 68641-1-Ig

    IHC staining of human colon cancer using 68641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68641-1-Ig (PSMB7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included
  • IHC staining of human colon cancer using 68641-1-Ig

    IHC staining of human colon cancer using 68641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68641-1-Ig (PSMB7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included
  • IHC staining of human skin cancer using 68641-1-Ig

    IHC staining of human skin cancer using 68641-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68641-1-Ig (PSMB7 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included

PSMB7 Monoclonal antibody

PSMB7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68641-1-Ig

产品说明书

CloneNo. 3D7B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Z, Proteasome subunit beta type-7, EC:3.4.25.1, 3D7B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, LNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMB7 Ready-To-Use IHC Kit. PSMB7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68641-1-Ig targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6544

Product name: Recombinant human PSMB7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-277 aa of BC000509

Sequence: MAAVSVYAPPVGGFSFDNCRRNAVLEADFAKRGYKLPKARKTGTTIAGVVYKDGIVLGADTRATEGMVVADKNCSKIHFISPNIYCCGAGTAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGTRLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 7
别名 Z, Proteasome subunit beta type-7, EC:3.4.25.1, 3D7B8
计算分子量29.9 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC000509
基因名称 PSMB7
Gene ID (NCBI) 5695
RRIDAB_3670385
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99436
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

实验方案

Product Specific Protocols
WB protocol for PSMB7 antibody 68641-1-IgDownload protocol
IHC protocol for PSMB7 antibody 68641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols