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验证数据展示

  • WB analysis using 32134-1-AP

    WB analysis using 32134-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 32134-1-AP

    WB analysis using 32134-1-AP

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 32134-1-AP

    IHC staining of human placenta using 32134-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 32134-1-AP

    IHC staining of human placenta using 32134-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCR/CD201 Polyclonal antibody

EPCR/CD201 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32134-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, bEnd.3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32134-1-AP targets EPCR/CD201 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein C receptor, endothelial
别名 Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41
计算分子量27kDa
观测分子量 27 kDa, 40-50 kDa
GenBank蛋白编号NM_011171.2
基因名称 PROCR
Gene ID (NCBI) 19124
RRIDAB_3670204
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ64695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424). EPCR is approximately 46 kDa, truncated EPCR is 23 kDa, truncated EPCR with the transmembrane domain is 26 kDa, and soluble EPCR is 43 kDa (PMID: 11686350).

实验方案

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 32134-1-APDownload protocol
IHC protocol for EPCR/CD201 antibody 32134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols