验证数据展示
产品信息
85244-4-PBS targets PRAS40 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag35446 Product name: Recombinant human PRAS40 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 131-256 aa of BC051844 Sequence: MDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | AKT1 substrate 1 (proline-rich) |
| 别名 | AKT1S1, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, Proline rich AKT1 substrate 1, Proline-rich AKT1 substrate 1 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC051844 |
| 基因名称 | PRAS40 |
| Gene ID (NCBI) | 84335 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96B36 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).
