验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, HEK-293T cells, mouse heart tissue, rat heart tissue, rat colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25157-1-AP targets PPP1R12C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PPP1R12C fusion protein Ag18559 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 12C |
| 别名 | Protein phosphatase 1 myosin-binding subunit p85, Protein phosphatase 1 myosin-binding subunit of 85 kDa, p84, MBS85, LENG3 |
| 计算分子量 | 782 aa, 85 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC008030 |
| 基因名称 | PPP1R12C |
| Gene ID (NCBI) | 54776 |
| RRID | AB_3669464 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZL4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP1R12C is a subunit of myosin phosphatase. PPP1R12C regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R12C antibody 25157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
