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验证数据展示

  • WB analysis using 25157-1-AP

    WB analysis using 25157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25157-1-AP (PPP1R12C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PPP1R12C Polyclonal antibody

PPP1R12C Polyclonal Antibody for WB, ELISA
Cat No. 25157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

LENG3, MBS85, p84, Protein phosphatase 1 myosin-binding subunit of 85 kDa, Protein phosphatase 1 myosin-binding subunit p85

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, HEK-293T cells, mouse heart tissue, rat heart tissue, rat colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25157-1-AP targets PPP1R12C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18559

Product name: Recombinant human PPP1R12C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 422-716 aa of BC008030

Sequence: GLLKTGSSGALGPPERRIAEGAPGAGLQRSASSSWLEGTSTQAKELRLARITPTPSPKLPEPSVLSEVTKPPPCLENSSPPSRIPEPESPAKPNVPTASTAPPADSRDRRRSYQMPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAEKAAGKAPESEKPAQSLDPSRRPRVPGVENSDSPAQREAPDGQGPGPQAAREHRKVGKEWRGPAEGEEAEPADRSQESSTLEGGPSARRQRWQRDLNPEPEPESEEPDGGFRTLYAELRRENERLREALTETTLRLAQLKVELE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 12C
别名 LENG3, MBS85, p84, Protein phosphatase 1 myosin-binding subunit of 85 kDa, Protein phosphatase 1 myosin-binding subunit p85
计算分子量 782 aa, 85 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC008030
基因名称 PPP1R12C
Gene ID (NCBI) 54776
RRIDAB_3669464
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R12C is a subunit of myosin phosphatase. PPP1R12C regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton.

实验方案

Product Specific Protocols
WB protocol for PPP1R12C antibody 25157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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