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验证数据展示

  • WB analysis using 31793-1-AP (same clone as 31793-1-PBS)

    WB analysis using 31793-1-AP (same clone as 31793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31793-1-AP (PON3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

PON3 Polyclonal antibody, PBS Only

PON3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31793-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31793-1-PBS targets PON3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36602

Product name: Recombinant human PON3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-167 aa of NM_000940.2

Sequence: AFRERVNASREVEPVEPENCHLIEELESGSEDIDILPSGLAFISSGLKYPGMPNFAPDEPGKIFLMDLNEQNPRAQALEISGGFDKELFNPHGISIFIDKDNTVYLYVVNHPHMKSTVEIFKFEEQQRSLVYLKTIKHELLKSVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paraoxonase 3
别名 Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1
计算分子量NM_000940.2 40kDa，354aa
观测分子量40 kDa
GenBank蛋白编号NM_000940.2
基因名称 PON3
Gene ID (NCBI) 5446
RRIDAB_3670115
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ15166
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.

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