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验证数据展示

  • WB analysis using 68263-1-Ig

    WB analysis using 68263-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 68263-1-Ig

    WB analysis of HEK-293 using 68263-1-Ig

    WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells.

  • FC experiment of HeLa using 68263-1-Ig

    FC experiment of HeLa using 68263-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug POLR3D Monoclonal antibody (68263-1-Ig, Clone:2F5G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

POLR3D Monoclonal antibody

POLR3D Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68263-1-Ig

产品说明书

CloneNo. 2F5G4

宿主/亚型

Mouse / IgG1

种属反应性

human, rabbit

应用

WB, FC (Intra), ELISA

别名

RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, rabbit brain tissue, K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLR3D Ready-To-Use IHC Kit. POLR3D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68263-1-Ig targets POLR3D in WB, FC (Intra), ELISA applications and shows reactivity with human, rabbit samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rabbit
免疫原

CatNo: Ag7646

Product name: Recombinant human POLR3D protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 45-398 aa of BC004484

Sequence: SRDLTLGGVKKKTFTPNIISRKIKEEPKEEVTVKKEKRERDRDRQREGHGRGRGRPEVIQSHSIFEQGPAEMMKKKGNWDKTVDVSDMGPSHIINIKKEKRETDEETKQILRMLEKDDFLDDPGLRNDTRNMPVQLPLAHSGWLFKEENDEPDVKPWLAGPKEEDMEVDIPAVKVKEEPRDEEEEAKMKAPPKAARKTPGLPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLTEGQVGKLLIRKSGRVQLLLGKVTLDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESLLDHKHR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
别名 RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC004484
基因名称 POLR3D
Gene ID (NCBI) 661
RRIDAB_2935348
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP05423
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

实验方案

Product Specific Protocols
WB protocol for POLR3D antibody 68263-1-IgDownload protocol
FC protocol for POLR3D antibody 68263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols