验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human skin tissue, human spleen tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PNPT1 Ready-To-Use IHC Kit. PNPT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14487-1-AP targets PNPT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PNPT1 fusion protein Ag5888 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polyribonucleotide nucleotidyltransferase 1 |
| 别名 | PNPase old 35, PNPase 1, OLD35, old 35, EC:2.7.7.8 |
| 计算分子量 | 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC053660 |
| 基因名称 | PNPT1 |
| Gene ID (NCBI) | 87178 |
| RRID | AB_2165820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| IHC protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| IF protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| IP protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis
| ||
Am J Hum Genet A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.
| ||
Proc Natl Acad Sci U S A EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells. | ||
PLoS Genet RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer. |
