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验证数据展示

  • WB analysis of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    WB analysis of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25469-1-AP (PNPLA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    IP experiment of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    IP result of anti-PNPLA4 (IP:25469-1-AP, 4ug; Detection:25469-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

PNPLA4 Polyclonal antibody, PBS Only

PNPLA4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 25469-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

Calcium-independent phospholipase A2-eta, DXS1283E, EC:3.1.1.3, EC:3.1.1.4, GS2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25469-1-PBS targets PNPLA4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22091

Product name: Recombinant human PNPLA4 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-253 aa of BC020746

Sequence: MKHINLSFAACGFLGIYHLGAASALCRHGKKLVKDVKAFAGASAGSLGASVLLTAPEKIEECNQFTYKFAEEIRRQSFGAVTPGYDFMARLRSGMESILPPSAHELAQNRLHVSITNAKTRENHLVSTFSSREGLIKVLLASSFVPIYAGLKLVEYKGQKWVDGGLTNALPILPVGRTVTISPFSGRLDISPQDKGQLDLYVNIAKQDIMLSLANLVRLNQALFPPSKRKMESLYQCGFDDTVKFLLKENWFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 patatin-like phospholipase domain containing 4
别名 Calcium-independent phospholipase A2-eta, DXS1283E, EC:3.1.1.3, EC:3.1.1.4, GS2
计算分子量 253 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC020746
基因名称 PNPLA4
Gene ID (NCBI) 8228
RRIDAB_2880095
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41247
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.

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