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PLCB2 Polyclonal antibody

PLCB2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27456-1-AP

Print datasheet

Synonyms

Phospholipase C beta 2, phospholipase C, beta 2, PLC beta 2, PLCB2

验证数据展示

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 27456-1-AP

    IF Staining of THP-1 using 27456-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PLCB2 antibody (27456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IF detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27456-1-AP targets PLCB2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen PLCB2 fusion protein Ag25771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, beta 2
Synonyms Phospholipase C beta 2, phospholipase C, beta 2, PLC beta 2, PLCB2
Calculated Molecular Weight 134 kDa
Observed Molecular Weight 134 kDa
GenBank Accession NumberBC000939
Gene Symbol PLCB2
Gene ID (NCBI) 5330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCB2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2) is also named as PLC-beta-2. PLCB2 is a critical regulator of platelet responses upon activation (PMID: 27465150). NF-κB regulates expression of PLC-β2. The PLCB2 genes was found to be differentially expressed in human breast cancer MCF-7 cells, and to be associated with multidrug resistance (PMID: 2951275). The knockdown of PLCB2 suppressed cell viability and promoted cell apoptosis by activating the Ras/Raf/MAPK pathway. Thus, PLCB2 may utilized as a potential therapeutic target in patients with melanoma (PMID: 31746389).

实验方案

Product Specific Protocols
WB protocol for PLCB2 antibody 27456-1-APDownload protocol
IF protocol for PLCB2 antibody 27456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols