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验证数据展示

  • WB analysis using 25799-1-AP

    WB analysis using 25799-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25799-1-AP (PLA2G12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 25799-1-AP

    WB analysis of HepG2 using 25799-1-AP

    WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HepG2 cells.

  • WB analysis of HuH-7 using 25799-1-AP

    WB analysis of HuH-7 using 25799-1-AP

    WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HuH-7 cells.

  • IHC staining of human colon cancer using 25799-1-AP

    IHC staining of human colon cancer using 25799-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25799-1-AP (PLA2G12B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

PLA2G12B Polyclonal antibody

PLA2G12B Polyclonal Antibody for WB, ELISA
Cat No. 25799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

FKSG71, Group XIIB secretory phospholipase A2-like protein, Group XIII secretory phospholipase A2-like protein, GXIIB, GXIII sPLA2 like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, HuH-7 cells, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25799-1-AP targets PLA2G12B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22877

Product name: Recombinant human PLA2G12B protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 115-195 aa of BC111946

Sequence: LDVCYDTCGANKYRCDAKFRWCLHSICSDLKRSLGFVSKVEAACDSLVDTVFNTVWTLGCRPFMNSQRAACICAEEEKEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group XIIB
别名 FKSG71, Group XIIB secretory phospholipase A2-like protein, Group XIII secretory phospholipase A2-like protein, GXIIB, GXIII sPLA2 like
计算分子量 195 aa, 22 kDa
观测分子量21-22 kDa
GenBank蛋白编号BC111946
基因名称 PLA2G12B
Gene ID (NCBI) 84647
RRIDAB_3085823
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BX93
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B is a secreted group XIIB secretory phospholipase A2-like protein belonging to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (PMID: 14516201). It is located outside the cell membranes and strongly expressed in liver, small intestine and kidney, and recently reports revealed that Pla2g12b gene may be implicated in HDL cholesterol levels and metabolism (PMID: 22912808). The calculated molecular weight of PLA2G12B is 21 kDa.

实验方案

Product Specific Protocols
WB protocol for PLA2G12B antibody 25799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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