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PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10101-2-AP

Print datasheet

Synonyms

hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP

验证数据展示

  • WB analysis of human placenta using 10101-2-AP

    WB analysis of human placenta using 10101-2-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 10101-2-AP

    IP experiment of A549 using 10101-2-AP

    IP result of anti-PITRM1 (IP:10101-2-AP, 4ug; Detection:10101-2-AP 1:500) with A549 cells lysate 2000ug.

  • IF Staining of A549 using 10101-2-AP

    IF Staining of A549 using 10101-2-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 10101-2-AP (PITRM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human breast cancer using 10101-2-AP

    IHC staining of human breast cancer using 10101-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included
  • IHC staining of human breast cancer using 10101-2-AP

    IHC staining of human breast cancer using 10101-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included

经过测试的应用

Positive WB detected inhuman placenta tissue, A549 cells, mouse testis tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PITRM1 Ready-To-Use IHC Kit. PITRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10101-2-AP targets PITRM1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PITRM1 fusion protein Ag0150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pitrilysin metallopeptidase 1
Synonyms hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP
Calculated Molecular Weight 60 kDa, 117 kDa
Observed Molecular Weight 110-117 kDa
GenBank Accession NumberBC001150
Gene Symbol PITRM1
Gene ID (NCBI) 10531
RRIDAB_2165145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.

实验方案

Product Specific Protocols
WB protocol for PITRM1 antibody 10101-2-APDownload protocol
IHC protocol for PITRM1 antibody 10101-2-APDownload protocol
IF protocol for PITRM1 antibody 10101-2-APDownload protocol
IP protocol for PITRM1 antibody 10101-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
View Article
mouseWB

PLoS One

Proteolysis controls endogenous substance p levels.

Authors - Mitchell Andrew J AJ
View Article