验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27903-1-AP targets PIK3AP1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Rat, Mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag27398 Product name: Recombinant human PIK3AP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 634-794 aa of BC029917 Sequence: LNQKKRSESFRFQQENLKRLRDSITRRQREKQKSGKQTDLEITVPIRHSQHLPAKVEFGVYESGPRKSVIPPRTELRRGDWKTDSTSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEVPEVTRSRSPGPPQVDGTPTMSLERPPRVPPRAASQRPPTRETF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase adaptor protein 1 |
| 别名 | PIK3AP1, RP11 34E5.3 |
| 计算分子量 | 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC029917 |
| 基因名称 | PIK3AP1 |
| Gene ID (NCBI) | 118788 |
| RRID | AB_3086007 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZUJ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), also known as B-cell adaptor for PI3K (BCAP), is a specific protein adaptor of B-cells that is expressed in hematopoietic cells. Besides, PIK3AP1 has been implicated in the development of gastric cancer. (PMID: 26121143, 28087538, 31078520)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIK3AP1 antibody 27903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
