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验证数据展示

  • WB analysis using 30527-1-AP

    WB analysis using 30527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 30527-1-AP

    IF Staining of SKOV-3 using 30527-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30527-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, rat heart tissue, SKOV-3 cells
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30527-1-AP targets PI16 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 PI16 fusion protein Ag32556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase inhibitor 16
别名 CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9
计算分子量 463 aa, 49 kDa
观测分子量 100 kDa, 49 kDa
GenBank蛋白编号BC022399
基因名称 PI16
Gene ID (NCBI) 221476
RRIDAB_3086350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).

实验方案

Product Specific Protocols
WB protocol for PI16 antibody 30527-1-APDownload protocol
IF protocol for PI16 antibody 30527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols