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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    WB analysis of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    IF Staining of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16481-1-AP (PHAX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

PHAX Polyclonal antibody, PBS Only

PHAX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16481-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16481-1-PBS targets PHAX in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHAX fusion protein Ag9602 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylated adaptor for RNA export
别名
计算分子量 394 aa, 44 kDa
观测分子量 55 kDa
GenBank蛋白编号BC021161
基因名称 PHAX
Gene ID (NCBI) 51808
RRIDAB_2299663
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Small nuclear and small nucleolar RNAs (snRNAs and snoRNAs) are essential components of snRNPs and snoRNPs, and have a critical role in the maturation of, respectively, mRNAs and rRNAs within the nucleus of eukaryotic cells. Complex and specific pathways exist for the assembly of snRNPs and snoRNPs, involving, nucleocytoplasmic transport of snRNAs and intranuclear transport between compartments of snoRNAs. In metazoa, a subset of spliceosomal snRNAs are exported from the nucleus after transcription. This export occurs in a large complex containing a snRNA, the nuclear cap binding complex (CBC), RanGTP, the leucine-rich nuclear export signal receptor CRM1/Xpo1, and the recently identified phosphoprotein PHAX (phosphorylated adaptor for RNA export). PHAX contains a conserved single-stranded nucleic acid binding domain (RNA_GG_bind domain) with no sequence homology with any other known RNA-binding module [PMID:15574332,11333016, 15574332]