验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30560-1-AP targets PGLS in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag30836 Product name: Recombinant human PGLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC014006 Sequence: MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 6-phosphogluconolactonase |
| 别名 | 6 phosphogluconolactonase, 6PGL, PGLS |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC014006 |
| 基因名称 | PGLS |
| Gene ID (NCBI) | 25796 |
| RRID | AB_3086363 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95336 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
6-phosphogluconolactonase (PGLS) ,the key enzyme of pentose phosphate pathway, was aberrantly highly expressed in various cancers, including cervical cancer, breast cancer, glioblastomas, and hepatic cancer, etc.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PGLS antibody 30560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
