验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26441-1-AP targets PET112L in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PET112L fusion protein Ag23740 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PET112-like (yeast) |
| 别名 | Glu ADT subunit B, HSPC199, PET112, PET112 like (yeast), PET112L |
| 观测分子量 | 55-62 kDa |
| GenBank蛋白编号 | BC136547 |
| 基因名称 | PET112L |
| Gene ID (NCBI) | 5188 |
| RRID | AB_3669534 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75879 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATB (Glutamyl-tRNA(Gln) amidotransferase subunit B), also called PET112L, is a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in the proper aminoacylation of mitochondrial glutaminyl mt-tRNA (mt-tRNA(Gln)). The other 2 subunits in the enzyme complex are GATA and GATC. The complex plays an essential role in mitochondrial translation and protein synthesis (PMID: 30283131, 19805282).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PET112L antibody 26441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |