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验证数据展示

  • IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

    IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66970-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

    IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66970-1-PBS in a different storage buffer formulation.

Nephrin Monoclonal antibody, PBS Only

Nephrin Monoclonal Antibody for IHC, ELISA
Cat No. 66970-1-PBS

产品说明书

CloneNo. 1D11H8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, ELISA

别名

NPHS1, Renal glomerulus-specific cell adhesion receptor, NPHS 1, NPHN, CNF

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66970-1-PBS targets Nephrin in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Nephrin fusion protein Ag19273 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nephrosis 1, congenital, Finnish type (nephrin)
别名 NPHS1, Renal glomerulus-specific cell adhesion receptor, NPHS 1, NPHN, CNF
计算分子量 1241 aa, 135 kDa
GenBank蛋白编号BC156935
基因名称 Nephrin
Gene ID (NCBI) 4868
RRIDAB_2882292
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60500
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).