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验证数据展示

  • WB analysis using 67921-1-Ig

    WB analysis using 67921-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67921-1-Ig (NUDT22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 67921-1-Ig

    FC experiment of Jurkat using 67921-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NUDT22 (67921-1-Ig, Clone:3C3H9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NUDT22 Monoclonal antibody

NUDT22 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67921-1-Ig

产品说明书

CloneNo. 3C3H9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

WB, ELISA, FC (Intra)

别名

UDPG pyrophosphatase, PP11246, NUDT 22, Nucleoside diphosphate-linked moiety X motif 22, EC:3.6.1.45

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67921-1-Ig targets NUDT22 in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag14787

Product name: Recombinant human NUDT22 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-303 aa of BC006129

Sequence: MDPEVTLLLQCPGGGLPQEQIQAELSPAHDRRPLPGGDEAITAIWETRLKAQPWLFDAPKFRLHSATLAPIGSRGPQLLLRLGLTSYRDFLGTNWSSSAAWLRQQGATDWGDTQAYLADPLGVGAALATADDFLVFLRRSRQVAEAPGLVDVPGGHPEPQALCPGGSPQHQDLAGQLVVHELFSSVLQEICDEVNLPLLTLSQPLLLGIARNETSAGRASAEFYVQCSLTSEQVRKHYLSGGPEAHESTGIFFVETQNVRRLPETEMWAELCPSAKGAIILYNRVQGSPTGAALGSPALLPPL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 22
别名 UDPG pyrophosphatase, PP11246, NUDT 22, Nucleoside diphosphate-linked moiety X motif 22, EC:3.6.1.45
计算分子量 303 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC006129
基因名称 NUDT22
Gene ID (NCBI) 84304
RRIDAB_2918673
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRQ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human NUDT22 belongs to the diverse NUDIX family of proteins, is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate.

实验方案

Product Specific Protocols
WB protocol for NUDT22 antibody 67921-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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