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验证数据展示

  • WB analysis of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    WB analysis of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 27430-1-AP (NODAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 27430-1-AP (same clone as 27430-1-PBS)

    FC experiment of HEK-293T using 27430-1-AP (same clone as 27430-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human NODAL (27430-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

NODAL Polyclonal antibody, PBS Only

NODAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 27430-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

nodal homolog (mouse), Nodal homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27430-1-PBS targets NODAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26729

Product name: Recombinant human NODAL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-347 aa of BC104976

Sequence: EWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nodal homolog (mouse)
别名 nodal homolog (mouse), Nodal homolog
计算分子量 347 aa, 40 kDa
观测分子量 45 kDa
GenBank蛋白编号BC104976
基因名称 NODAL
Gene ID (NCBI) 4838
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96S42
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nodal is a member of the transforming growth factor-β (TGF-β) superfamily that plays critical roles during embryogenesis. Nodal also regulates cell proliferation, apoptosis, and invasion in cancer cells. However, it appears to exert both tumor-suppressing and tumor-promoting effects, depending on the cell type (PMID: 22645523). Nodal signals through ALK7 receptor to inhibit proliferation and to induce apoptosis in ovarian cancer cell lines and breast cancer cell lines (PMID: 15531507, PMID: 21383881).

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