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验证数据展示

  • WB analysis using 29319-1-AP

    WB analysis using 29319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29319-1-AP (NLGN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29319-1-AP

    IHC staining of mouse brain using 29319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29319-1-AP

    IHC staining of mouse brain using 29319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NLGN3 Polyclonal antibody

NLGN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29319-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29319-1-AP targets NLGN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NLGN3 fusion protein Ag30536 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroligin 3
别名 NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1
计算分子量 94 kDa
观测分子量90-110 kDa
GenBank蛋白编号BC051715
基因名称 NLGN3
Gene ID (NCBI) 54413
RRIDAB_3086116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuroligin3 (NLGN3) belongs to the neuroligin family, a class of postsynaptic cell adhesion molecules that regulate synapse organization and dendritic outgrowth, and NLGN3 missense variants have previously been associated with autistic spectrum disorder (ASD).

实验方案

Product Specific Protocols
WB protocol for NLGN3 antibody 29319-1-APDownload protocol
IHC protocol for NLGN3 antibody 29319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols