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NEK5 Polyclonal antibody

NEK5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26254-1-AP

Print datasheet

Synonyms

NEK5, NimA related protein kinase 5

验证数据展示

  • WB analysis using 26254-1-AP

    WB analysis using 26254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26254-1-AP (NEK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26254-1-AP

    IHC staining of human thyroid cancer using 26254-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26254-1-AP (NEK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26254-1-AP targets NEK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen NEK5 fusion protein Ag24419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 5
Synonyms NEK5, NimA related protein kinase 5
Calculated Molecular Weight 708 aa, 81 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC063885
Gene Symbol NEK5
Gene ID (NCBI) 341676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P3R8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEK5 antibody 26254-1-APDownload protocol
IHC protocol for NEK5 antibody 26254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols