• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 21497-1-AP (same clone as 21497-1-PBS)

    WB analysis using 21497-1-AP (same clone as 21497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21497-1-AP (NEBL antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    IF Staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using NEBL antibody (21497-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

NEBL Polyclonal antibody, PBS Only

NEBL Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21497-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

21497-1-PBS targets NEBL in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NEBL fusion protein Ag15882 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nebulette
别名 Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein
计算分子量 1014 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC110453
基因名称 NEBL
Gene ID (NCBI) 10529
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76041
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.