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验证数据展示

  • WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

    WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

    WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

    WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS5 antibody included
  • Featured Product
  • KD/KO Validated

NDUFS5 Polyclonal antibody, PBS Only

NDUFS5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15224-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NDUFS5 Ready-To-Use IHC Kit. NDUFS5 primary antibody included.

产品信息

15224-1-PBS targets NDUFS5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7441

Product name: Recombinant human NDUFS5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-106 aa of BC001884

Sequence: MPFLDIQKRFGLNIDRWLTIQSGEQPYKMAGRCHAFEKEWIECAHGIGYTRAEKECKIEYDDFVECLLRQKTMRRAGTIRKQRDKLIKEGKYTPPPHHIGKGEPRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
别名
计算分子量 13 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001884
基因名称 NDUFS5
Gene ID (NCBI) 4725
RRIDAB_2149021
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43920
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).

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