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NBCn1 Polyclonal antibody

NBCn1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29442-1-AP

Print datasheet

Synonyms

Bicarbonate transporter, BT, DKFZp686H168, NBC2, NBC2B, NBC3, NBCn1, SBC2, SLC4A6, SLC4A7

验证数据展示

  • WB analysis using 29442-1-AP

    WB analysis using 29442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29442-1-AP (NBCn1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inBGC-823 cells, HEK-293 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29442-1-AP targets NBCn1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen NBCn1 fusion protein Ag30545 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms Bicarbonate transporter, BT, DKFZp686H168, NBC2, NBC2B, NBC3, NBCn1, SBC2, SLC4A6, SLC4A7
Calculated Molecular Weight 136 kDa
Observed Molecular Weight 136 kDa
GenBank Accession NumberNM_003615
Gene Symbol SLC4A7
Gene ID (NCBI) 9497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6M7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC4A7 also known as NBCn1, NBC3, NBC2, is an electroneutral Na(+)/HCO(3)(-) cotransporter (PMID: 14736710). SLC4A7 mediates the coupled movement of sodium and bicarbonate ions across the plasma membrane. SLC4A7 plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis (PubMed:29779931). SLC4A7 is expressed in testis, spleen, and retina. SLC4A7 may be a major regulator of extracellular pH of retina where light stimulation produces an extracellular alkalization and may contribute as a solute transporter to the prevention of retinal detachment(PMID: 9610397).

实验方案

Product Specific Protocols
WB protocol for NBCn1 antibody 29442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols