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验证数据展示

  • WB analysis using 32022-1-AP

    WB analysis using 32022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32022-1-AP (NAXE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 32022-1-AP

    IF Staining of A549 using 32022-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NAXE antibody (32022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse cerebellum using 32022-1-AP

    IHC staining of mouse cerebellum using 32022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32022-1-AP (NAXE antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAXE Polyclonal antibody

NAXE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AIBP, AI-BP, APOA1BP, Apolipoprotein A-I-binding protein, EC:5.1.99.6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue, rat kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32022-1-AP targets NAXE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NAXE fusion protein Ag35306 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein A-I binding protein
别名 AIBP, AI-BP, APOA1BP, Apolipoprotein A-I-binding protein, EC:5.1.99.6
计算分子量32 kDa
观测分子量29 kDa
GenBank蛋白编号NM_144772.2
基因名称 APOA1BP
Gene ID (NCBI) 128240
RRIDAB_3670173
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8NCW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAD(P)H-hydrate epimerase (NAXE, also known as AIBP, YJEFN1, and APOA1BP) catalyzes the interconversion between the S- and R-forms of NAD(P)HX, and essentially supplies the substrate for the stereospecific enzyme NAD(P)HX dehydratase (NAXD) (PMID: 35866541). NAXE variants can cause neurometabolic disorder by disrupting the cellular NAD(P)HX repair system (PMID: 31745726; 27616477).

实验方案

Product Specific Protocols
WB protocol for NAXE antibody 32022-1-APDownload protocol
IHC protocol for NAXE antibody 32022-1-APDownload protocol
IF protocol for NAXE antibody 32022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols