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验证数据展示

  • WB analysis using 23892-1-AP

    WB analysis using 23892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23892-1-AP (N4BP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 23892-1-AP

    IHC staining of rat testis using 23892-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23892-1-AP (N4BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

N4BP2 Polyclonal antibody

N4BP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23892-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

B3BP, KIAA1413, NEDD4 binding protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23892-1-AP targets N4BP2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag20943

Product name: Recombinant human N4BP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1426-1770 aa of BC126466

Sequence: SVMERQRQEEVSCGKFMQDPSLVGHTGLDNPEQKSSQRTGKKLLKTLTASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHNLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTGQKSKEKKPKKLKETEETPSELSFQDFEYPDYDDYRAEAFLHQQKRMECYSKAKEAYRIGKKNVATFYAQQGTLHEQKMKEANHLAAIEIFEKVNASLLPQNVLDLHGLHVDEALEHLMRVLEKKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLISHSFRFSEIKPGCLKVMLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NEDD4 binding protein 2
别名 B3BP, KIAA1413, NEDD4 binding protein 2
计算分子量 1770 aa, 199 kDa
观测分子量199 kDa
GenBank蛋白编号BC126466
基因名称 N4BP2
Gene ID (NCBI) 55728
RRIDAB_3669422
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ86UW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP2 (Nedd4 binding protein 2) is a Bcl-3 binding protein, which contains a polynucleotide kinase domain (PNK) at the N-terminus and a Small MutS Related (Smr) domain with nicking endonuclease activity near C-terminus. N4BP2 was at higher levels in a majority of tumor tissues examined, relative to paired normal tissues (PMID: 17626640).

实验方案

Product Specific Protocols
IHC protocol for N4BP2 antibody 23892-1-APDownload protocol
WB protocol for N4BP2 antibody 23892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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