验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G66321-1-5C targets Fascin in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag6074 Product name: Recombinant human Fascin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-187 aa of BC000521 Sequence: MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human Fascin (1B5G3) |
| 别名 | 55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL |
| 计算分子量 | 55 kDa |
| GenBank蛋白编号 | BC000521 |
| 基因名称 | Fascin |
| Gene ID (NCBI) | 6624 |
| ENSEMBL Gene ID | ENSG00000075618 |
| RRID | AB_3673947 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGGCCGTTCGCGGTACGCCCATATAAGAAA |
| 条形码序列 | GCCGTTCGCGGTACG |
| 形式 | Liquid |
| UNIPROT ID | Q16658 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |
