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验证数据展示

  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G65204-1-5C

产品说明书

CloneNo. UCHT4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65204-1-5C targets CD8 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 Thymocytes and Sézary T cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD8 (UCHT4)
别名 CD8, CD8A, CD8a molecule, Leu2, MAL, p32
计算分子量 235 aa, 26 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_3673935
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTGGAATCTAATACCACCCATATAAGAAA
条形码序列TGGAATCTAATACCA
形式 Liquid
UNIPROT IDP01732
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol