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MultiProTM 5CFLX Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

TGGAATCTAATACCA

CloneNo.

UCHT4

Cat No : G65204-1-5C

Print datasheet

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

验证数据展示

  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65204-1-5C on Resting PBMC

    G65204-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65204-1-5C on PBMC treated with 4hr LPS + TI

    G65204-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65204-1-5C targets CD8 in applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell
Tested Reactivity Human
Immunogen Thymocytes and Sézary T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Full Name MultiProTM 5CFLX Anti-Human CD8 (UCHT4)
Synonyms CD8, CD8A, CD8a molecule, Leu2, MAL, p32
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTGGAATCTAATACCACCCATATAAGAAA
Barcode SequenceTGGAATCTAATACCA
Form Liquid
UNIPROT IDP01732
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol