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验证数据展示

  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G65254-1-5C

产品说明书

CloneNo. E11

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65254-1-5C targets CD35 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD35 (E11)
别名 C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN
计算分子量 224 kDa
GenBank蛋白编号NM_000573
基因名称 CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_3673940
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGAATGAATTGGATTCGCCCATATAAGAAA
条形码序列AATGAATTGGATTCG
形式 Liquid
UNIPROT IDP17927
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol